Growing support for dietary restriction

A new study led by Harvard T.H. Chan School of Public Health (HSPH) researchers identifies a key molecular mechanism behind the health benefits of dietary restriction, or reduced food intake without malnutrition. Also known as calorie restriction, dietary restriction is best known for its ability to slow aging in laboratory animals.

The findings show that restricting two amino acids, methionine and cysteine, results in increased production of hydrogen sulfide (H₂S) and protection against ischemia-reperfusion injury — the damage to tissue that occurs following the interruption of blood flow, such as during organ transplants and stroke. Increased H₂S production upon dietary restriction was also associated with lifespan extension in worms, flies, and yeast.

Although H₂S gas is extremely toxic in high amounts, low levels present in naturally occurring sulfur springs have long been associated with health benefits. Mammalian cells also produce low levels of H₂S, but this is the first time that this molecule has been linked directly to the health benefits of dietary restriction.

“This finding suggests that H₂S is one of the key molecules responsible for the benefits of dietary restriction in mammals and lower organisms as well,” said senior author James Mitchell, associate professor of genetics and complex diseases. “While more experiments are required to understand how H₂S exerts its beneficial effects, it does give us a new perspective on which molecular players to target therapeutically in our efforts to combat human disease and aging.”

The study appears online today in Cell.

Dietary restriction is a type of intervention that can include reduced overall food intake, decreased consumption of particular macronutrients such as protein, or intermittent bouts of fasting. It is known to have beneficial health effects, including protection from tissue injury and improved metabolism. It has also been shown to extend the lifespan of multiple model organisms, ranging from yeast to primates. The molecular explanations for these effects are not completely understood, but were thought to require protective antioxidant responses activated by the mild oxidative stress caused by dietary restriction itself.

First author Christopher Hine, research fellow in the Department of Genetics and Complex Diseases, and colleagues demonstrated that one week of dietary restriction increased antioxidant responses and protected mice from liver ischemia-reperfusion injury, but surprisingly, this protective effect was intact even in animals that could not mount such an antioxidant response. Instead, the researchers found that the protection required increased production of H₂S, which occurred upon reduction of dietary intake of methionine and cysteine. When the diet was supplemented with these two amino acids, increased H₂S production and dietary restriction benefits were both lost.

The investigators also found that genes involved in H₂S production were also required for longevity benefits of dietary restriction in other organisms.

“These findings give us a better understanding of how dietary interventions extend lifespan and protect against injury. More immediately, they could have important implications for what to eat and not to eat before a planned acute stress like surgery, when the risk of ischemic injury can be relatively high,” said Hine.

Other Harvard T.H. Chan School of Public Health authors include Eylul Harputlugil, Lear Brace, Humberto Trevino Villarreal, Pedro Mejia, Yue Zhang, and William Mair.